- ￥5000
- GeneTex
- 美国
- GTX12134
- 2025年07月11日
- WB, ICC/IF, IHC-P, FACS, IP, MS
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX12134
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, MS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IKB alpha
- 抗体英文名：
IKB alpha antibody [6A920]
- 抗体名：
IKB alpha 抗体 [6A920]
- 规格：
50 μg
IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920].
Dilution : 0.5 μg/ml
FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 0.25μg/10^6 cells
WB analysis of 100 μg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody.
*ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation
WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].
Dilution : 2 μg/ml
WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].
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文献和实验【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
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