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Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat, Zebrafish
12 months from the shipping date of the product.
Human
GTX121184
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, EM
1 mg/ml(Please refer to the vial label for the specific concentration.)
Histone H3K27me3 (Tri-methyl Lys27)
Histone H3K27me3 (Tri-methyl Lys27) antibody
Histone H3K27me3 (Tri-methyl Lys27) 抗体
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: PFA-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) dilution: 1:100.
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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