- ￥1700 - 4000
- GeneTex
- 美国
- GTX117114
- 2025年07月12日
- WB, ICC/IF, IHC-P, IHC-Wm
- Rabbit
- Human, Mouse, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX117114
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Wm
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
LIN7A
- 抗体英文名：
LIN7A antibody [N1C3]
- 抗体名：
LIN7A 抗体 [N1C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sample (30 μg of whole cell lysate)
A: zebrafish eye
12% SDS PAGE
GTX117114 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using LIN7A antibody [N1C3] (GTX117114) at 1:300 dilution.
LIN7A antibody [N1C3] detects Lin7a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
LIN7A antibody [N1C3] (GTX117114) dilution: 1:200.
Sample (30 μg of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX117114 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LIN7A(GTX117114) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
LIN7A antibody [N1C3] detects LIN7A protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LIN7A protein stained by LIN7A antibody [N1C3] (GTX117114) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献和实验Yoohwa Chung et al., Biochem Biophys Rep 2020 (PMID:33319072)
Kamijo A et al., Histochem Cell Biol 2019 (PMID:31410570)
Silvia Jordans et al., Mol Neurobiol 2022 (PMID:35445918)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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