- ￥1700 - 4000
- GeneTex
- 美国
- GTX116041
- 2025年07月09日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX116041
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1.87 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
- 抗体英文名：
H-Ras antibody
- 抗体名：
H-Ras 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody (GTX116041) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with H-RAS antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed HeLa, using H-RAS (GTX116041) antibody at 1:200 dilution.
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文献和实验Sugita S et al., Int J Oncol 2018 (PMID:29901113)
Sarin N et al., Int J Mol Sci 2018 (PMID:29518977)
Brandt LP et al., PLoS One 2017 (PMID:28982163)
Tsuboi M et al., J Biol Chem 2017 (PMID:28912272)
Antonella Catalano et al., Cancers (Basel) 2021 (PMID:33924486)
Hu W et al., JCI Insight 2021 (PMID:33400686)
Wu HT et al., Am J Chin Med 2020 (PMID:32668964)
Brandt LP et al., Oncotarget 2018 (PMID:29731980)
Gao P et al., Breast Cancer Res 2018 (PMID:30208932)
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Ras是大鼠肉瘤(rat sarcoma,Ras)的英文缩写。Ras蛋白是原癌基因 c—ras的表达产物，相对分子质量为21kDa，属单体 GTP结合蛋白，具有弱的 GTP酶活性。Ras蛋白的活性状态对细胞的生长、分化、细胞骨架、蛋白质运输和分泌等都具有影响，其活性则是通过与GTP或GDP的结合进行调节。 Ras的活性受两个蛋白的控制,一个是鸟苷交换因子(guanine nucleotide exchange factor, GEF),它的作用是促使GDP从Ras蛋白
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