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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human RAB6A Recombinant protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX110646
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.21 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RAB6A
- 抗体英文名:
RAB6A antibody
- 抗体名:
RAB6A 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RAB6A antibody detects RAB6A protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA at RT for 15 min.
Green: RAB6A protein stained by RAB6A antibody (GTX110646) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(GTX110646) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(GTX110646) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
RAB6A antibody detects RAB6A protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (GTX110646) dilution: 1:1000
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文献和实验Ishida M et al., J Cell Biol 2019 (PMID:31575603)
Wu CH et al., Sci Rep 2017 (PMID:29127306)
Lee PL et al., Elife 2015 (PMID:25821985)
Kawabata M et al., PLoS Pathog 2021 (PMID:33760868)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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