- ￥1700 - 4000
- GeneTex
- 美国
- GTX105870
- 2025年07月07日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX105870
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
MDH2
- 抗体英文名：
MDH2 antibody
- 抗体名：
MDH2 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX105870 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MDH2 antibody detects MDH2 protein by western blot analysis.
A. 50 μg rat lung lysate/extract
10% SDS-PAGE
MDH2 antibody (GTX105870) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
MDH2 antibody (GTX105870) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MDH2 antibody detects MDH2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
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文献和实验May EW et al., Pharmacol Res 2014 (PMID:25242635)
Li JM et al., Environ Mol Mutagen 2013 (PMID:23813585)
Marco Rosina et al., Cell Metab 2022 (PMID:35305295)
Yi-Wen Lo et al., Journal of Cellular and Molecular Medicine 2015 (PMID:25639359)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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