- ￥1700 - 4000
- GeneTex
- 美国
- GTX103042
- 2025年07月14日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat, African green monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, African green monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX103042
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377384)
- 抗体英文名：
Cyclin A2 antibody
- 抗体名：
Cyclin A2 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (GTX103042) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.
Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Pan J et al., Oncogene 2010 (PMID:20802531)
Gupta RK et al., EMBO J 2022 (PMID:36161661)
Cardile A et al., Int J Mol Sci 2023 (PMID:36674794)
Jennifer P Ditano et al., Cell Cycle 2021 (PMID:34156324)
San-Yuan Chen et al., Oncol Lett 2022 (PMID:35069870)
Zanr? V et al., Int J Mol Sci 2022 (PMID:35163058)
Hinds JW et al., ACS Pharmacol Transl Sci 2021 (PMID:34423276)
Pietil? EA et al., Nat Commun 2021 (PMID:34162871)
Javary J et al., Liver Int 2021 (PMID:33792165)
Fu H et al., Mol Med Rep 2021 (PMID:33200803)
Zhang G et al., Eur J Pharmacol 2020 (PMID:32950500)
Huang HY et al., J Cell Mol Med 2020 (PMID:32672400)
Ogawa M et al., Oncotarget 2019 (PMID:30719233)
Honda T et al., Sci Rep 2020 (PMID:31980707)
Yeh HT et al., Eur J Pharmacol 2019 (PMID:31518562)
Chen SY et al., Int J Mol Med 2019 (PMID:31485612)
Liu WL et al., Molecules 2019 (PMID:30824767)
Liu YH et al., Phytomedicine 2018 (PMID:30097117)
Huang CY et al., Cells 2018 (PMID:30544747)
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Li JM et al., Ther Adv Med Oncol 2018 (PMID:30159048)
Honda T et al., J Cell Physiol 2018 (PMID:30066954)
Liang HH et al., Life Sci 2018 (PMID:30056019)
Suk FM et al., Sci Rep 2018 (PMID:29426877)
Fu H et al., Mol Med Rep 2018 (PMID:29344642)
Kokabu S et al., J Biol Chem 2017 (PMID:28607151)
Liu YH et al., Sci Rep 2017 (PMID:28134285)
Lin IL et al., Cancer Cell Int 2014 (PMID:24393431)
Szu-Ting Lin et al., Cellular and Molecular Life Sciences 2015 (PMID:25596698)
Li LH et al., PLoS One 2014 (PMID:25105411)
Chen Y et al., Biochim Biophys Acta 2012 (PMID:22200450)
大鼠腺苷 A2b 受体 (A2b R)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 A2b R 单抗包被于酶标板上，标准品和样品中的 A2b R 与单抗结合，加入生物素化的抗大鼠 A2b R ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸
大鼠腺苷 A2b 受体 (A2b R)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 A2b R 单抗包被于酶标板上，标准品和样品中的 A2b R 与单抗结合，加入生物素化的抗大鼠 A2b R ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
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