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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101777
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP, IHC, PLA
- 浓度:
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
FEN1
- 抗体英文名:
FEN1 antibody
- 抗体名:
FEN1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX101777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FEN1 protein by 4 μg FEN1 antibody (GTX101777)
10 % SDS-PAGE
The immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FEN1 antibody detects FEN1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: FEN1 stained by FEN1 antibody (GTX101777) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Immunofluorescence analysis of PFA-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.
FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FEN1 antibody (GTX101777) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Szczesny B et al., Sci Rep 2016 (PMID:27808278)
Ying Ma et al., Antioxidants (Basel) 2023 (PMID:36829835)
Zeng X et al., FASEB J 2019 (PMID:31266372)
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