- ￥1700 - 4000
- GeneTex
- 美国
- GTX101777
- 2025年07月13日
- WB, ICC/IF, IHC-P, IP, IHC, PLA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101777
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, IHC, PLA
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
FEN1
- 抗体英文名：
FEN1 antibody
- 抗体名：
FEN1 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX101777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FEN1 protein by 4 μg FEN1 antibody (GTX101777)
10 % SDS-PAGE
The immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FEN1 antibody detects FEN1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: FEN1 stained by FEN1 antibody (GTX101777) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Immunofluorescence analysis of PFA-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.
FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FEN1 antibody (GTX101777) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Szczesny B et al., Sci Rep 2016 (PMID:27808278)
Ying Ma et al., Antioxidants (Basel) 2023 (PMID:36829835)
Zeng X et al., FASEB J 2019 (PMID:31266372)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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