- ￥8300
- GeneTex
- 美国
- GTX28934
- 2025年07月15日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Rabbit
- Human, Mouse, Rat, Bovine, Dog, Hamster, Pig
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- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide: MVDTESPICPLSPLEADD, corresponding to N terminal amino acids 1-18 of Mouse PPAR alpha.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Dog, Hamster, Pig
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX28934
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度：
0.98 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PPAR alpha
- 抗体英文名：
PPAR alpha antibody
- 抗体名：
PPAR alpha 抗体
- 规格：
100 μg
Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20ºC. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20ºC. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody (GTX28934). Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 μg/mL overnight at 4ºC. Secondary antibody: Alexa FluorR 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa FluorR 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.
Affinity Purified anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 μg of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Immunohistochemistry using GeneTex's anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
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文献和实验Nehmi VA et al., J Integr Med 2021 (PMID:34108131)
Kuo MW et al., J Biomed Sci 2021 (PMID:33423678)
Cizkova K et al., Toxicol Appl Pharmacol 2018 (PMID:29960002)
Grossini E et al., J Endocrinol 2014 (PMID:24860147)
Cizkova K et al., Biology (Basel) 2022 (PMID:36101378)
Cizkova K et al., Biomedicines 2021 (PMID:34572440)
PPAR-Alpha Cloning, Expression, and Characterization
Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays
Estimation of the PPAR Agonism of Fibrates by a Combined MM-Docking Approach
Fibrates are peroxisome proliferator-activated alpha receptor (PPARα) activators derived from fibric acid and are the most clinically used therapeutics in the treatment of hypertriglyceridemia. Recently, we reported a computational approach
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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