- ￥1700 - 4000
- GeneTex
- 美国
- GTX101055
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101055
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SEC13L1
- 抗体英文名：
SEC13L1 antibody [N1C3]
- 抗体名：
SEC13L1 抗体 [N1C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1(GTX101055) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SEC13L1 antibody [N1C3] detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% PFA at RT for 15 min.
Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:500.
Blue: Hoechst 33342 staining.
SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 μg of SEC13L1 antibody (GTX101055) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (GTX101055) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Lingyun Long et al., Nature 2021 (PMID:34795452)
Wolfson RL et al., Nature 2017 (PMID:28199306)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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