- ￥1700 - 4000
- GeneTex
- 美国
- GTX100622
- 2025年07月15日
- WB, ICC/IF, FACS, IP
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100622
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS, IP
- 浓度：
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
OCT4
- 抗体英文名：
Oct4 antibody
- 抗体名：
Oct4 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX100622) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX100622) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100622)antibody at 1:50 dilution(green) or rabbit IgG (black).
Immunofluorescence analysis of PFA-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:200 dilution.
Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX100622 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% PFA at RT for 15 min.
Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.
Immunofluorescence analysis of PFA-fixed Human ESC, using Oct4(GTX100622) antibody at 1:400 dilution.
Immunofluorescence analysis of PFA-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:100 dilution.
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文献和实验O'Day E et al., J Biol Chem 2015 (PMID:26045559)
Chen PM et al., Neoplasia 2014 (PMID:25425970)
Blanas A et al., Cancers (Basel) 2020 (PMID:32927726)
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
recombination). Transgenes have been generated using different regions of the Oct4 gene promoter to drive expression of either lacZ or GFP, which allows the activity of the Oct4 promoter to be followed in living cells and tissues. The bacterial LacZ gene encodes
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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