Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX100531
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, IP, ChIP assay
浓度:
0.68 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
MDM2
抗体英文名:
MDM2 antibody
抗体名:
MDM2 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% PFA at RT for 15 min. Green: MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.
Untreated (–) and treated (+) HCT116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples: Jurkat whole cell extract A. 30 μg Jurkat cell whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody (GTX100531) 7.5 % SDS-PAGE The immunoprecipitated MDM2 protein was detected by MDM2 antibody (GTX100531) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Application Reference
Liu Y et al., J Immunol Res 2015 (PMID:26090506)
Chai YR et al., Scand J Immunol 2014 (PMID:24965442)
Zeng M et al., Cancer Manag Res 2020 (PMID:33061576)
Zheng H et al., J Dent Res 2019 (PMID:31847675)
Soyoung I. et al., Int J Clin Exp Pathol 2018;11(4)
Tseng CW et al., Cancer Res 2018 (PMID:29599405)
Briest F et al., Neuroendocrinology 2017 (PMID:28910819)