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- Cell Signaling Technology
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- 2025年07月30日
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文献和实验Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
and splicing in whole-cell extracts and were fairly inefficient, because of the different optima for these two reactions (1 ,2 ). It was later shown that these reactions could be uncoupled (3 ,4 ), but obtaining discrete pre-mRNAs in useful amounts remained
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell cycle in eukaryotic cells. One of the widely used assays to understand the complex mammalian replication system is the cell-free in vitro replication assay (IVRA
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