Phospho-Ezrin (Thr567)/Radixin

3141:

Immunofluorescence , Western Blotting

Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at threonine 567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-treated, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody (left) or Ezrin/Radixin/Moesin Antibody #3142 (right).

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

• Wu, K. L. et al. (2004) The NHE1 Na/H Exchanger Recruits Ezrin/Radixin/Moesin Proteins to Regulate Akt-dependent Cell Survival. J. Biol. Chem. 279 (25), 26280-26286. Applications: Western Blotting
• John, G. R. et al. (2004) Interleukin-1beta Induces a Reactive Astroglial Phenotype via Deactivation of the Rho GTPase-Rock Axis. J. Neurosci. 24 (11), 2837-2845. Applications: Western Blotting
• Fievet, B. T. et al. (2004) Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin. J. Cell Biol. 164 (5), 653-659. Applications: Western Blotting
• Ren, L. et al. (2009) Oncogene 28, 792-802. Applications: Western Blotting
• Li, Y. et al. (2007) J Immunol 178, 1938-47. Applications: Western Blotting
• Chiba, H. et al. (2006) J Cell Biol 175, 971-80. Applications: Western Blotting
• Coffey, G.P. et al. (2009) J Cell Sci 122, 3137-44. Applications: Western Blotting
• Duterme, C. et al. (2009) J Biol Chem 284, 33495-508. Applications: Western Blotting
• Canals, D. et al. (2010) J Biol Chem 285, 32476-85. Applications: Western Blotting
• Baeyens, N. et al. (2010) Am J Physiol Cell Physiol 299, C1530-40. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 3142 Ezrin/Radixin/Moesin Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 3144 Phospho-Ezrin (Tyr353) Antibody
• 3145 Ezrin Antibody
• 3146 Moesin Antibody
• 3150 Moesin (Q480) Antibody

For Research Use Only. Not For Use In Diagnostic Procedures.