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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
Methods to Detect Protein Glutathionylation
) Primary antibody against protein of interest and corresponding species‐specific HRP‐conjugated secondary antibody, 1:1000 dilution in TBS‐T
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
. Designing heterodimeric two‐stranded alpha‐helical coiled‐coils: Effects of hydrophobicity and alpha‐helical propensity on protein folding, stability, and specificity. J. Biol. Chem. 277:37272‐37279.
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