- ¥5000
- Biosensis
- 3.9 ng/mL - 200 ng/mL
- BEK-2233-1P
- 2025年08月27日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
研卉生物
- 检测范围：
3.9 ng/mL - 200 ng/mL
- 检测方法：
ELISA
- 规格：
1 x 96 pre
Description
| Human LR³ insulin-like Growth Factor-I (LR³IGF-I) was developed by GroPep Bioreagents (www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR³IGF-I Rapid™ ELISA kit combines GroPep’s many years of expertise in the field of IGF research and Biosensis’ newly established Rapid™ ELISA platform. This collaboration has resulted in the new LR³IGF-I Rapid™ ELISA kit, which provides for the sensitive, specific and reliable quantification of LR³IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³IGF-I protein, a biotinylated anti- LR³IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR³IGF-I present in samples and the supplied protein standard. This LR³IGF-I RapidTM ELISA kit has been developed, optimized and validated to quantify LR³IGF-I protein in cell culture medium. It is likely to be used to measure LR³IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
|
| Batch No. | Refer to the product label. |
| Antigen | LR³IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. |
| Other Names | Human LR3 insulin-like Growth Factor-I. |
| Accession | Q9NO10 IGF1_HUMAN (unmodified protein) |
| Specificity | Human |
| Cross-reactivity | The assay is intended for quantification of LR³IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%. |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, wash and sample buffers and detailed protocols. |
| Range | 3.9 ng/mL - 200 ng/mL |
| Sensitivity | Typical limit of detection (LOD) for LR³IGF-I is 1 ng/mL determined as 150% of the blank value. |
 |
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay. |
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
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