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48
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研卉生物
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1-4周
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48支/盒
15 ml - 50 µl
Available with modified regenerated cellulose membrane; Hydrosart®
- Highest recovery at low absorption (95-98%)
- Extremely short concentration time (30-fold in 15 min.)
- Very small holdup volume (< 20 µl)
- Crystal clear housing and volume graduations
- Vertical twin membrane for ultra fast concentration
- Safe sample concentration due to dead-stop volume
Description
Vivaspin 15R is the latest member of the Vivaspin product family with all the unique features of Sartorius Stedim Biotech centrifugal concentrators including a patented vertical twin membrane and a dead stop. Vivaspin 15R is targeting the volume segment of 2 to 15 ml with the Hydrosart® regenerated cellulose membrane. This membrane is ideal when extremely high recovery rate and very low adsorption is required, for example in applications such as concentration and desalting of immunoglobulin fractions.
Rotor Compatibility
Please note: Vivaspin 15R (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes.
Performance Characteristics
| Time to concentrate up to 30x [min.] at 20°C and solute recovery % |
||||
|---|---|---|---|---|
| Rotor | Swing Bucket | 25° Fixed angle | ||
| Start volume | 15 ml | 12.5 ml | ||
| Min. | Rec. | Min. | Rec. | |
| Insulin chain A 0.1 mg/ml* (2,500 MW) | ||||
| 2,000 MWCO | 70 | 96 % | 60 | 96 % |
| Aprotinin 0.1 mg/ml* (6,500 MW) | ||||
| 5,000 MWCO | 47 | 95 % | 45 | 95 % |
| Cytochrome c 0.25 mg/ml* (12,400 MW) | ||||
| 5,000 MWCO | 45 | 96 % | 45 | 96 % |
| 10,000 MWCO | 25 | 94 % | 18 | 94 % |
| a-chymotrypsin 0.25 mg/ml* (25,000 MW) | ||||
| 5,000 MWCO | 50 | 98 % | 45 | 98 % |
| 10,000 MWCO | 25 | 98 % | 18 | 98 % |
| Ovalbumin 1.0 mg/ml* (45,000 MW) | ||||
| 10,000 MWCO | 20 | 98 % | 14 | 98 % |
| 30,000 MWCO | 15 | 94 % | 12 | 94 % |
| BSA 1.0 mg/ml* (66,000 MW) | ||||
| 30,000 MWCO | 18 | 98 % | 15 | 98 % |
| IgG 0.1 mg/ml* in DMEM (160,000 MW) | ||||
| 30,000 MWCO | 30 | 98 % | 25 | 96 % |
proteins other than IgG made up in 50 mM potassium sulphate, 150 mM sodium chloride, pH 7.4
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