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GFP羊驼纳米抗体偶联agar
ose beads限时大特价1500元GFP羊驼纳米抗体偶联agarose beads
Anti-GFP tag nanobody (VHH, Single Domain antibody) conjugated Agarose Beads
GFP标签融合蛋白
50% slurry
Immunoprecipitation (IP), 20μL of gel slurry. 2 μg of GFP can be precipitated with 10 μL of 50% gel slurry. Optimal working dilutions must be determined by end user.
羊驼
all species
琼脂糖微球
单克隆
4度
液体
VHH, Single Domain antibody
GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
1mL
Form of Antibody: | Agarose beads Conjugated anti-GFP Nanobody, in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 0.02% sodium azide as preservative. 50% suspension, total beads suspension volume of 1000 µL (500 µL packed beads) |
Bead size: | ~ 90 µm (cross-linked 8% agarose beads) |
Specificity: | This antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein. |
UniProt ID: | P42212 |
Reactivity: | All Species Expected |
Tested applications: | Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), Chromatin Immunoprecipitation (ChIP) In IP, 25 µL of beads suspension for ~1000 µg of crude total protein solution. Optimal dilutions/concentrations should be determined by the end user. |
Binding capacity: | 10 µg of GFP can be precipitated with 25 µL of beads suspension. |
Host: | Alpaca |
Clonality: | Monoclonal, nanobody |
Isotype: | Alpaca single domain antibody (nanobody, VHH) |
Immunogen: | Fusion protein corresponding to Aequorea victoria GFP aa 1-246. |
Positive Controls: | exogenously expressed GFP proteins in cells or E. coli. |
Storage instruction: | Store at +4°C and do not freeze. |
Expiration Date: | Expires one year from date of receipt when stored as instructed |
Buffer | Composition | |
Lysis buffer (Co-IP) | 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40 | |
RIPA buffer | 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.1% SDS; 1% Triton X-100; 1% Deoxycholate | |
Dilution/Wash buffer | 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA | |
Glycine-elution buffer | 200 mM glycine pH 2.5 | |
2 x SDS-sample buffer | 120 mM Tris/Cl pH 6.8; 20% glycerol; 4% SDS, 0.04% bromophenol blue; 10% β-mercaptoethanol |
Product | Cat. no. |
Anti-Myc tag Mouse mAb | AT0023 |
Anti-HA tag Mouse mAb | AT0024 |
Anti-His tag Mouse mAb | AT0025 |
Anti-GST tag Mouse mAb | AT0027 |
Anti-GFP tag Mouse mAb | AT0028 |
Anti-V5 tag Mouse mAb | AT0026 |
Anti-RFP tag Mouse mAb | AT0038 |
Protein A/G Agarose Beads for IP/CoIP | IF0001 |
Protein A/G Magnetic Beads for IP/CoIP | IF0002 |
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资料下载:
AT1765-GFP-Catch-Aga说明书GFP羊驼纳米抗体偶联agarose beads (100uL-0.5mL-1mL) - engibody.pdf 附件下载 (下载 0 次)
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