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50 µg/1 mg
| 规格: | 50 µg | 产品价格: | ¥3870 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥29480 |
蛋白名称:IL-6R蛋白, IL-6R protein
蛋白构建:A DNA sequence encoding the mouse IL6Rα (NP_034689.2) extracellular domain (Ile 44-Glu 357) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 20
蛋白分子量:The recombinant mouse IL6Rα comprises 349 amino acids with a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_034689.2
蛋白氨基酸序列:Ile44-Glu357
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验CUT&Tag(Cleavage Under Targets and Tagmentation)【1】是一种研究蛋白质-DNA 相互作用的新技术。与传统的染色质免疫沉淀(Chromatin Immunoprecipitation, ChIP)相比,CUT&Tag 具有信噪比高、可重复性好、实验周期短(1 天实现从细胞到文库构建)、细胞投入量低等显著优势。该方法一经问世,便受到广大科研工作者的青睐。 在过去的一年时间里,科研工作者在动物、植物细胞中成功应用 CUT&Tag
of downstream signaling pathways. Key to understanding this process is the study of the protein–protein interactions involving R proteins. Recognition and signaling mechanisms are mediated by both intramolecular interactions that take place between different
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪
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