Recombinant Mouse VCAM1 / CD106 Protein (His & Fc Tag) | 重组小鼠 VCAM1 / CD106 蛋白 (His & Fc标签)
- ￥2570
- Sino Biological
- 北京
- 50163-M03H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse VCAM1 / CD106 Protein (His & Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：VCAM1蛋白, VCAM1 protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1-coated plates (10 μg/ml, 100 μg/well), approximately >70% cells will adhere specifically.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Phe 25
蛋白分子量：The recombinant mouse VCAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 922 amino acids and has a predicted molecular mass of 102 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1/Fc monomer is approximately 110-120 kDa due to glycosylation.
蛋白NP号：NP_035823.3
蛋白氨基酸序列：Met1-Glu698
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验。[37] 使用这种方法，可以检测活小鼠胃肠道内重组的发光沙门氏菌的定殖和不同的喂食物质对其的影响。[38] 肿瘤研究 与药物功效研究相同，肿瘤的生长与转移，可以通过活体中注射BL重组肿瘤细胞，再成像进行监测。[39] 另一种方式，初期的肿瘤和不确定的转移可以通过使用基因工程的发光细胞作为探针进行定位。[40] a human colon carcinoma xenograft 模式中已经开始使用双标记，一个标记检测特定基因的转录活性，另一个组成性表达，作为一个内参照物。[41] 细胞
Intracellular Cytokine Staining Protocol
that the investigators evaluate the use and efficacy of different protein transport inhibitors in their specific assay system.Note: Generally, the non-specific background staining of fixed and permeabilized cells is higher than surface staining; therefore, extra protein
Intracellular Cytokine Staining Protocol
of the stimulation. It is advised that the investigators evaluate the use and efficacy of different protein transport inhibitors in their specific assay system. Note: Generally, the non-specific background staining of fixed and permeabilized cells is higher
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