重组小鼠 BACE-1蛋白 (His 标签)

小鼠 BACE1蛋白|BACE1 protein|BACE1(Mouse, C-His)（产品说明）
蛋白名称：BACE1蛋白, BACE1 protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Thr 457) of mouse BACE1 (NM_011792.5) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is >2 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 22
蛋白分子量：The secreted recombinant mouse BACE1 consists of 447 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NM_011792.5
蛋白氨基酸序列：Met1-Thr457
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.