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100 µg
蛋白名称:14-3-3 beta蛋白, 14-3-3 beta protein
蛋白构建:A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 93 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_003395.1
蛋白氨基酸序列:Met1-Asn246
蛋白标签:N-cleavage
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
debris. 13. Measure OD420 and OD550. 14. Calculate Units using the following formula: U= 1000 x [(OD420)-(1.75 x OD550)] / [(Time) x (Vol) x OD600] Where Vol is volume of culture used in assay in mls, and Time is minutes at 30 deg C
Introduction of human 14-3-3 proteins
-3-3 proteins are cytosolic,a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes.Using a panel of isoform-specific antisera we find that the epsilon,eta,gamma,beta,and zeta isoforms are all present
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