- ￥1010 - 5170
- Sino Biological
- 北京
- 11675-V08B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant H1N1(A/Puerto Rico/8/34/Mount Sinai)NP(I116M)Protein(His)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/50.00 µg/100.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥1010.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥1400.0 |
| 规格： | 100.00 µg | 产品价格： | ￥5170.0 |
H1N1 Nucleocapsid/NP蛋白|Nucleocapsid/NP protein|Nucleocapsid/NP(H1N1, C-His)（产品说明）
蛋白名称：Nucleocapsid/NP蛋白, Nucleocapsid/NP protein
蛋白构建：A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (AAM75159.1) (Met1-Gly490) was fused with a polyhistidine tag at the C-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met 1
蛋白分子量：The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：AAM75159.1
蛋白氨基酸序列：Met1-Gly490
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Nucleocapsid/NP蛋白, Nucleocapsid/NP protein
蛋白构建：A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (AAM75159.1) (Met1-Gly490) was fused with a polyhistidine tag at the C-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met 1
蛋白分子量：The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：AAM75159.1
蛋白氨基酸序列：Met1-Gly490
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Henry, C; et al. Influenza Virus Vaccination Elicits Poorly Adapted B Cell Responses in Elderly Individuals. Cell host & microbe, PMID: 30795982
2, Tan, HX; et al. Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Frontiers in immunology, PMID: 30984186
3, Wu, K; et al. Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1. ACS applied materials & interfaces, PMID: 32150378
4, Marinaik, CB; et al. Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants. Cell Reports Medicine, PMID: 32984856
5, Luetkens, T; et al. Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19. Blood advances, PMID: 33031540
6, Vuorela, A; et al. Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1. Nature communications, PMID: 33863907
7, Taheri, M; et al. Toward a universal influenza virus vaccine: Some cytokines may fulfill the request. Cytokine, PMID: 34555604
8, Vanderven, HA; et al. Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence. Immunology and cell biology, PMID: 34687553
9, Tan, HX; et al. Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles. Science immunology, PMID: 35089815
10, Gregoire, C; et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity, PMID: 35768001
11, Hong, D; et al. Absorption-Modulated SiO2@Au Core-Satellite Nanoparticles for Highly Sensitive Detection of SARS-CoV-2 Nucleocapsid Protein in Lateral Flow Immunosensors. ACS applied materials & interfaces, PMID: 36191048 1
2, Yari, P; et al. Magnetic Particle Spectroscopy for Point-of-Care: A Review on Recent Advances. Sensors (Basel, Switzerland), PMID: 37177614 1
3, Hong, D; et al. Plasmonic Approach to Fluorescence Enhancement of Mesoporous Silica-Coated Gold Nanorods for Highly Sensitive Influenza A Virus Detection Using Lateral Flow Immunosensor. ACS nano, PMID: 37595106 1
4, Hanson, CH; et al. CD62L expression marks a functionally distinct subset of memory B cells. Cell reports, PMID: 38060451 1
5, Ko, KH; et al. A vaccine platform targeting lung-resident memory CD4+ T-cells provides protection against heterosubtypic influenza infections in mice and ferrets. Nature communications, PMID: 39609429 1
6, Kocoglu, MH; et al. Coordinated antiviral immune response in a patient with myeloma and systemic adenovirus infection post-BCMA CAR T cells. Blood advances, PMID: 39058975
2, Tan, HX; et al. Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Frontiers in immunology, PMID: 30984186
3, Wu, K; et al. Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1. ACS applied materials & interfaces, PMID: 32150378
4, Marinaik, CB; et al. Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants. Cell Reports Medicine, PMID: 32984856
5, Luetkens, T; et al. Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19. Blood advances, PMID: 33031540
6, Vuorela, A; et al. Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1. Nature communications, PMID: 33863907
7, Taheri, M; et al. Toward a universal influenza virus vaccine: Some cytokines may fulfill the request. Cytokine, PMID: 34555604
8, Vanderven, HA; et al. Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence. Immunology and cell biology, PMID: 34687553
9, Tan, HX; et al. Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles. Science immunology, PMID: 35089815
10, Gregoire, C; et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity, PMID: 35768001
11, Hong, D; et al. Absorption-Modulated SiO2@Au Core-Satellite Nanoparticles for Highly Sensitive Detection of SARS-CoV-2 Nucleocapsid Protein in Lateral Flow Immunosensors. ACS applied materials & interfaces, PMID: 36191048 1
2, Yari, P; et al. Magnetic Particle Spectroscopy for Point-of-Care: A Review on Recent Advances. Sensors (Basel, Switzerland), PMID: 37177614 1
3, Hong, D; et al. Plasmonic Approach to Fluorescence Enhancement of Mesoporous Silica-Coated Gold Nanorods for Highly Sensitive Influenza A Virus Detection Using Lateral Flow Immunosensor. ACS nano, PMID: 37595106 1
4, Hanson, CH; et al. CD62L expression marks a functionally distinct subset of memory B cells. Cell reports, PMID: 38060451 1
5, Ko, KH; et al. A vaccine platform targeting lung-resident memory CD4+ T-cells provides protection against heterosubtypic influenza infections in mice and ferrets. Nature communications, PMID: 39609429 1
6, Kocoglu, MH; et al. Coordinated antiviral immune response in a patient with myeloma and systemic adenovirus infection post-BCMA CAR T cells. Blood advances, PMID: 39058975
相关实验
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
磷酸化就在有丝分裂、细胞死亡、DNA 损伤修复、DNA 复制和重组过程中发挥着直接的作用。 组蛋白翻译后修饰多发生在组蛋白的 N-端尾部，包括甲基化、乙酰化、磷酸化、ADP-核糖基化、泛素化和小分子泛素化修饰，这些修饰有助于其他蛋白质与 DNA 的结合，从而产生协同或者拮抗作用来调控基因转录。例如，乙酰化使组蛋白尾部正电荷减少，从而削弱了与带负电荷 DNA 骨架的作用，而促进染色质呈开放状态, 甲基化激活或抑制基因功能主要依赖于修饰的位点，主要与赖氨酸残基的单甲基化、双甲基化或三甲基化有关。 组蛋白修饰最基本
扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性
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重组H1N1(A/Puerto Rico/8/34/Mount Sinai)NP(I116M)蛋白(His标签)
￥1010 - 5170
