- ￥2310 - 25190
- Sino Biological
- 北京
- 50036-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse CD16-2 / FCGR4 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥25190.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：FCGR4蛋白, FCGR4 protein
蛋白构建：The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 (Cat:50036-M08H) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:10702-HNAC). The EC50 of human IgG1 (Fc) (Cat:10702-HNAC) is 0.11-0.25 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 19
蛋白分子量：The recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
蛋白NP号：NP_653142.2
蛋白氨基酸序列：Met1-Gln203
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
，亲合力增高Kd为10-8M。IgG与Clq结合点位于CH2功能区中最后一个β折叠股318～322位氨基酸残基(Glu-x-Lys-x-Lys)。IgM倍以上。人类天然的抗A和抗B血型抗体为IgM，血型不符合引韦的输血反应发生快而且严重。 2.凝聚的IgA、IgG4和IgE等可通过替代途径活化补体。 (三)结合Fc受体 不同细胞表面具有不同Ig的Fc受体，分别用FcγR、FcεR、FcαR等来表示。当Ig与相应抗原
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