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rabbit
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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
10 mM Tris-HCl, pH 8.0 1 mM EDTA Forward Kinase Buffer (10X) 50 mM DTT 0.1 M MgCl2 Do not store
Angiotensin Protein Kinase Assay
on your needs. Don't skimp too much on the amount of S. aureus bugs that you use for precipitation or you will risk losses. 1. Immunoprecipitate the protein from 3 x 106 cells using 45 microliters S. aureus bugs. Resuspend the immunoprecipitates
the GE InCell 3000 automated imaging platform. The assay utilized a genetically modified HeLa cell line stably expressing mitogen-activated, protein-activating protein kinase-2 fused to enhanced green fluorescent protein (MK2–EGFP) and measured
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