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文献和实验甲状腺C细胞分泌的多肽激素。血浆CT浓度最高为每毫升50微微克。CT主要通过抑制骨吸收而降低血钙和血磷水平。大剂量CT的作用,即刻出现破骨细胞活动减弱并抑制破骨细胞生成,几小时后,成骨细胞活动增强,由于溶骨过程减弱,成骨过程增强,因而从骨释放入血的钙、磷减少。CT降低血钙的效应只持续数日。CT的长时间效应是溶骨和成骨过程都受抑制(由于破骨细胞减少,使成骨细胞也相应减少),因而对血钙水平无明显影响,只抑制骨更新。CT抑制肾小管重吸收钙、磷、钠、氯。在成人,CT调节血钙水平的作用
In Situ Hybridization Using Digoxigenin Labeled Probes
In Situ Hybridization Using Digoxigenin Labeled Probes The Cytogenetics Core performs polytene chromosome in situ hybridizations to polytene chromosomes to serve the needs of the BDGP's various projects. The Core prepares probes
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
extension cycle at 72 ˚C for 5 min. PCR reactions were performed in Gene Amp® PCR System 480 (Perkin-Elmer, Norwalk, CT, USA). DNA samples of normal subjects (blood donors) were included as well as negative control (with no DNA sample) in each reaction.
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