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文献和实验Too many bands on a Western blot
and/or proteins. Change of the blocking conditions may remedy the problem. Concentration of antigen too low . The resolution of SDS-PAGE is limited to 50-100 bands. If the relative concentration of the antigen of interest is too low
In Situ Hybridization Using Digoxigenin Labeled Probes
In Situ Hybridization Using Digoxigenin Labeled Probes The Cytogenetics Core performs polytene chromosome in situ hybridizations to polytene chromosomes to serve the needs of the BDGP's various projects. The Core prepares probes
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
DETECTION OF ß-GALACTOSIDASE The E. coli lacZ gene encoding ß-galactosidase (ß-gal) is the classical
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