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mouse
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北京鼎国
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文献和实验The production of monoclonal antibody by hybridoma
and grow up for ascites/ large flask production and further freeze down. Troubleshooting I strongly reccommend to use Southern Biotech Goat anti-Mouse Ig (H+L
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
Conjugation of monoclonal antibodies
Conjugation of monoclonal antibodies Prelude: You are free to copy and distribute these documents at will--but please do so in their entirety
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