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文献和实验Pharmacogenetics of ATP-Binding Cassette Transporters and Clinical Implications
Drug resistance is a severe limitation of chemotherapy of various malignancies. In particular efflux transporters of the ATP-binding cassette family such as ABCB1 (P-glycoprotein), the ABCC (multidrug resistance-associated protein) family
Introduction to the EMSA (Gel Shift) Technique
of DNA, requiring the DNA to be labeled before performing the experiment. Traditionally, DNA is radiolabeled with 32 P by incorporating an [a -32P]dNTP during a 3´ fill-in reaction using Klenow fragment or by 5´ end labeling using [g -32P]ATP and T
for every experiment (Bluescript is a nice control because it will turn blue on xgal/IPTG plates).If you assume the efficiency of the cells is between 107 and 108/µg of DNA,then you need to dilute the plasmid to a concentration of 0.1 ng/µl.Then transform 1 ml (0.1 ng
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