- ￥2310 - 3870
- Sino Biological
- 北京
- 10483-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Cathepsin B / CTSB Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3870.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human Cathepsin B / CTSB Protein (His Tag)
蛋白构建：A DNA sequence encoding the pre pro form of human CTSB (Arg18-Ile339) (NP_001899.1) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008) . The specific activity is >2,500 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Arg 18 & Phe 74
蛋白分子量：The recombinant human CTSB existing as the proform consists of 332 amino acids and has a predicted molecular mass of 37.2 kDa. rhCTSB migrates with the molecular weight of 36 and 43 kDa as the proform and mature form rspectively in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001899.1
蛋白氨基酸序列：Arg18-Ile339
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zhang D, et al. Transferrin receptor targeting chimeras for membrane protein degradation.Nature, PubMed ID: 39322661
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
) was exchanged with the multiple cloning site of pET15b (sequences between the Xba I to Bpu 1102I sites). The HMK coding sequence was then inserted by recombinant PCR directly downstream of and in frame with the His tag
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