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100 µg
蛋白名称:Cyclin A1蛋白, Cyclin A1 protein
蛋白构建:A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 (Cat:11013-H07B) at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1 (Cat:10739-H09B). The EC50 of biotinylated human CDK1 (Cat:10739-H09B) is 0.02-0.04 μg/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 (Cat:11013-H07B) at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His (Cat:10624-H08B). The EC50 of biotinylated human CDK2-His (Cat:10624-H08B)is 0.07-0.15 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:His
蛋白分子量:The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_003905.1
蛋白氨基酸序列:Met1-Gln465
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验带 EndNote X6/7 的文件响应缓慢甚至假死问题? A11: 由于 Word 中某一项拼写和语法检查功能与 EndNote 冲突,依次打开在 Word 中点击"文件"-「选项」-「校对」-「在 Word 文档中更正拼写和语法时」标签,取消选中「键入时标记语法错误」即可。 Q12:如何快速判断测序得到的序列方向并批量删除载体序列? A12:将测序得到的序列(大于 800bp 的需先拼接)和参考序列文件均以 FASTA 格式合并在一个文件内,先在 MEGA 多重
, YWHAZ. Cell Cycle Proteins Regulated by the Erk1/2 Pathway: CCNA1, CCNA2, CCNB1, CCNB2, CCND1, CCND2, CCND3, CCNE1, CCNE2, CDK2, CDK4, CDK6, CDKN1A, CDKN1B, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, E2F1, RB1. 2。T细胞与B细胞活化基因芯片 T细胞与B细胞活化基因芯片列有适应性免疫
3.5,会导致镍离子脱落 策略:改变洗脱办法,咪唑竞争性洗脱 可能原因:蛋白已沉淀在柱上 策略:减少上样量,或使用咪唑的线性梯度而不是分步洗脱以降低蛋白的浓度。试用去污剂或改变NaCl 的浓度,或在变性条件( 去折叠) 下洗脱( 用4-8 M 脲,或4-6 M 盐酸胍)。 可能原因:非特异性疏水或其他相互反应 策略:加非离子去污剂到洗脱缓冲液(如:2% Triton X-100)或增加NaCl 的浓度。 His 标签蛋白洗脱后杂带
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