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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within human MEK1 (MAPKK1) .
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5 % BSA with 0.09% sodium azide. Antibody was purified by immunogen-affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within human MEK1 (MAPKK1) .
- 目录编号:
252148
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
MEK1 (MAPKK1)
- 抗体英文名:
MEK1 (MAPKK1) Antibody
- 抗体名:
MEK1 (MAPKK1) Antibody
- 规格:
0.1 mg
MEK1 (MAPKK1) Antibody产品详细介绍:
| 产品名称: | MEK1 (MAPKK1) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252148 |
| 规格: | 0.1 mg |
| 产品描述: | MEK1 (MAPKK1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEKI (MAPKK1) activates ERK1 and ERK2 MAP kinases, enzymes which are involved in numerous signal transduction pathways. Defects in MEK1 (MAPKK1) are a cause of cardiofaciocutaneous syndrome (CFC syndrome), also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within human MEK1 (MAPKK1) . |
| 适用物种: | H |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:1,000-1:100,000; WB: 1:100-1:1,1000; IHC: 1:50-1:250 |
| 别名: | MAPKK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK1; MAP2K1; PRKMK1 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5 % BSA with 0.09% sodium azide. Antibody was purified by immunogen-affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验MAPK signaling pathway - Homo sapiens (human)
5, that are activated by specific MAPKKs: MEK1/2 for ERK1/2, MKK3/6 for the p38, MKK4/7 (JNKK1/2) for the JNKs, and MEK5 for ERK5. Each MAPKK, however, can be activated by more than one MAPKKK, increasing the complexity and diversity of MAPK signalling. Presumably
Western时,您会得到一个量化值,哪些组分占多少。Sampler Kit包含细胞质MEK1/2、线粒体凋亡诱导因子(AIF)、组蛋白H3和细胞骨架波形蛋白的兔单克隆抗体,以及山羊抗兔IgG。 研究人员也可利用试剂盒中的建议,或搜索文献,组装自己的Western blot或ELISA试剂盒。不过Cormier也提醒,并非每个抗体都有着它应有的特异性。 下游的分析 与超速离心相比,基于试剂盒的细胞分级分离方法快速又方便,也适合多个下游应用,如EMSA、报告基因分析、酶活
2结构域的接头蛋白(如Grb2)→鸟嘌呤核苷酸释放因子(如SOS)→Ras蛋白→MAPKKK(如Raf1)→MAPKK→MAPK→转录因子等(图21-24)。 图21-24 EGF受体介导的信号转导过程 表皮生长因子与受体结合后,可以使受体发生二聚体化,从而改变了受体的构象,使其中的蛋白酪氨酸激酶活性增强,受体自身的酪氨酸残基发生磷酸化,磷酸化的受体便形成了与含SH2结构域的蛋白分子Grb2结合的位点,导致Grb2与受体的结合。Grb2中有两个SH3结构域,该部位与一种称为
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