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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-M.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H, M, R, B
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
NF-M
- 抗体英文名:
NF-M Antibody
- 抗体名:
NF-M Antibody
- 规格:
0.1 mg
NF-M Antibody产品详细介绍:
| 产品名称: | NF-M Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/nf-m-antibody【参考图片】 |
| 货号: | 250771 |
| 规格: | 0.1 mg |
| 产品描述: | NF-M (Neurofilament medium polypeptide) is a neurofilament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. NF-M is phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to inhibition of polymerization. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-M. |
| 适用物种: | H, M, R, B |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名: | NF-M; Neurofilament triplet M; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; NEFM; NEF3; NFM |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验owen79 各位大侠好,小弟愚问一下,我想测定NF-KB活化后细胞核内的量,这需要提取核蛋白,但是蛋白量太少,提取出来能否测出NF-KB啊!thank you ! ronaldo_zj 1. 通过提取细胞核、细胞浆内的蛋白来行western blot检测NF-kappa B 的表达情况是可以的,因为很多时候是通过NF-kappa B转位来实现活化的,所以提取的时候我不知道楼主是自己配制试剂提取还是用试剂盒提取的,我建议
pmlt 我想做nf-kb的激活,请问如果我只用western 做Ikba和p-Ikba的半定量检测可以么? 细胞因子 可以,做完WB后用Image软件经行成像分析,可以半定量检测。园子里有很多这方面的帖子,你可以看看。 doctormy pmlt wrote: 我想做nf-kb的激活,请问如果我只用western 做Ikba和p-Ikba的半
superskyfly 我自己都觉得我自己很牛,千百人都做出来的试验,我就是做不出来。质粒用的是Promega p4.32-NF-kB-Luc, 转染用的是Invitrogen的Lipofactamin, 激活用的是Sigma生产的PMA和PHA-P,Assay用的是Promega dual,结果加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 用过PC3, Jurkat, A549,HCT116都是一样。
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