该产品被引用文献(仅展示部分): 1、Wang H; Chen Z; Wang M, et al., The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice. Foods 2022, 11 (12), 1747. PubMed: 35741945 2、Liu S; Meng F; Zhang D, et al., Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation. Front Nutr 2022, 9, 853225. PubMed: 35356725 3、Cai J; Yang J; Chen X, et al., Melatonin ameliorates trimethyltin chloride-induced cardiotoxicity: The role of nuclear xenobiotic metabolism and Keap1-Nrf2/ARE axis-mediated pyroptosis. Biofactors 2021. PubMed: 34570919 4、Liu S; Yu J; Fu M, et al., Regulatory effects of hawthorn polyphenols on hyperglycemic, inflammatory, insulin resistance responses, and alleviation of aortic injury in type 2 diabetic rats. Food Res Int 2021, 142, 110239. PubMed: 33773689 5、Liu S; Chang X; Yu J, et al., Cerasus humilis Cherry Polyphenol Reduces High-Fat Diet-Induced Obesity in C57BL/6 Mice by Mitigating Fat Deposition, Inflammation, and Oxidation. J Agric Food Chem 2020, 68 (15), 4424-4436. PubMed: 32227855
操作流程:
一、试剂配制: 试剂一应用液 : 用时取0.1ml加双蒸水至10ml,现用现配,需多少配多少,4°C保存。 试剂二 : 先向甲粉试剂中加入90-100°C的热双蒸水(50T 加入85ml;100T 加入170ml),充分完全溶解。然后将 已配好的甲液与乙液充分混合。此为过饱和溶液,室温静置冷却后,如有结晶,则取上清进行实验,4°C或室温保存6个 月。 试剂三应用液:用时加双蒸水进行溶解(50T 加入100ml;100T 加入200ml),避光4°C保存6个月。 试剂四应用液:用时加双蒸水50ml进行溶解,避光4°C保存6个月。 试剂五应用液:用时每支加双蒸水10ml溶解,避光4°C可保存5天。 GSH标准品溶剂应用液:按GSH标准品溶剂贮备液:双蒸水=1 : 9 即10倍稀释配成应用液,现用现配,4°C保存。 10mmol/L GSH标准品溶液的配制:GSH的分子量为307,每次测定前将10mg的GSH标准品加入到3.25ml的 GSH标准品溶剂应用液中,混匀,现用现配。 1mmol/L标准品溶液的配制:取10mmol/L GSH标准品溶液1ml加入GSH标准品溶剂应用液9ml,现用现配。 20µmol/L GSH标准品溶液:取10mmol/L GSH标准品溶液0.02ml加入GSH标准品溶剂应用液9.98ml,现用现配。 二、样本前处理。 a. 血清(浆):直接取原液进行检测。 b. 全血:取肝素抗凝全血20µl , 加双蒸水稀释至1ml,配成1:49的溶血液,充分混匀直至透亮为止。 c. 组织:准确称取组织重量,按重量(g):体积(ml)=1 : 9的比例,加入9倍体积的生理盐水,冰水浴条件下机械 匀浆,2500rpm离心10min,取上清液进行测定。 d. 细胞:将培养细胞消化,1000-1500rpm离心10min,弃上清,加0.3-0.5ml生理盐水制备成细胞悬液再进行破碎 (匀浆器匀浆、超声粉碎器或反复冻融3次进行破碎)。 e. 培养上清:悬浮细胞需1000-1500rpm离心10min,收集上清进行测定;贴壁细胞可直接吸取上清进行测定。 三、最佳取样量的参考值。 (1)血清(浆)样本:取待测血清(浆)0.1ml。
1、Wang H; Chen Z; Wang M, et al., The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice. Foods 2022, 11 (12), 1747. PubMed: 35741945 2、Liu S; Meng F; Zhang D, et al., Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation. Front Nutr 2022, 9, 853225. PubMed: 35356725 3、Cai J; Yang J; Chen X, et al., Melatonin ameliorates trimethyltin chloride-induced cardiotoxicity: The role of nuclear xenobiotic metabolism and Keap1-Nrf2/ARE axis-mediated pyroptosis. Biofactors 2021. PubMed: 34570919 4、Liu S; Yu J; Fu M, et al., Regulatory effects of hawthorn polyphenols on hyperglycemic, inflammatory, insulin resistance responses, and alleviation of aortic injury in type 2 diabetic rats. Food Res Int 2021, 142, 110239. PubMed: 33773689 5、Liu S; Chang X; Yu J, et al., Cerasus humilis Cherry Polyphenol Reduces High-Fat Diet-Induced Obesity in C57BL/6 Mice by Mitigating Fat Deposition, Inflammation, and Oxidation. J Agric Food Chem 2020, 68 (15), 4424-4436. PubMed: 32227855