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- 细胞类型：
cervix carcinoma
- 物种来源：
human
DSMZ no.:ACC 57
Species:human (Homo sapiens)
Cell type:cervix carcinoma
Origin: established from the epitheloid cervix carcinoma of a 31-year-old black woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid, continuously cultured human cell line
Biosafety level:1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:A
DSMZ Cell Culture Data:
Morphology: epithelial-like cells growing in monolayers
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids (cells also grow well in 90-95% RPMI 1640 + 5-10% h.i. FBS)
Subculture:
split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA; cells reach confluence quickly; seed out at ca. 1-2 x 106 cells/80 cm2
Incubation: at 37 °C with 5% CO2
Doubling time:ca. 48 hours
Harvest:cell harvest of ca. 5-15 x 106 cells/175 cm2
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
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文献和实验HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studies of the cell cycle
Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
1951年由 G． O． Gey等从人子宫颈癌组织分离出来的株细胞。 HeLa是原患者姓氏的缩写。这种细胞是现有来自人体的组织培养中最早的分离细胞，在世界各地的研究室继续培养，广泛应用于各种研究。它们在体外容易增殖形成上皮性的细胞排列。染色体数频率分布式为 78— 80，移植于人体皮下则形成肿瘤，在豚鼠的眼前房，大鼠的颊囊以及经 X射线皮质酮处理的小鼠可作异种移植，由此可以认为仍保持着癌的性状。广泛被用作分析细胞营养要求、细胞增殖和各种细胞化学的研究材料。易从细胞群落作无性
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