- ¥240
- 易欣生物
- 上海
- CC501-01
- 2026年01月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃
- 保质期：
6个月
- 英文名：
JM110 Chemically Competent Cell
- 库存：
现货
- 供应商：
上海易欣生物科技有限公司
- 规格：
10×100ul
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文献和实验Transforming chemically competent cells
: According to the original TSS paper and qualitative experience (JM), this step is completely optional and may actually reduce transformation efficiency. Incubate cells on ice for 2 min. Add 1 mL SOC (2XYT and LB are also suitable, original paper suggests
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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