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文献和实验Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
into each microcentrifuge tube (~500 islets/tube/mouse). Allow islets to settle to the bottom of the microcentrifuge tubes. Draw one islet pellet into a P200 pipetman (set at 130ul) using a straight thin-wall pipet tip. Place a silicone tube adapter
Please make sure to reference Andrew Hamilton for this protocol Probes : I use single stranded, 32 P labelled RNA probes transcribed in vitro from plasmid templates. Initially, you should use
SSR GEL and Silver Staining Protocol
for the silver staining work as well. The burst-pack's eliminate the c hemical weighing and mixing, deionizing, filtering and degassing steps. IV. GEL POURING: 1. Immediately prior to pouring, add 500 ul 10% ammonium persulfate (0.1 g + 1 ml d.i. water
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