- 询价
- Thermo scientific -fermentas
- EP0441
- 2025年10月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 5X Reaction Buffer | ||||
| EP0441 | 10,000 u (200 u/µl) (for 50 reactions of 20 µl) |
1.00 ml | EP0441 | |
| EP0442 | 5 x 10,000 u (200 u/µl) (for 250 reactions of 20 µl) |
5 x 1.00 ml | EP0442 | |
| EP0447 | 2000 u (200 u/µl) (for 10 reactions of 20 µl) |
1.00 ml |
- Features
-
- 高效制备全长的第一链cDNA，最长可达13 kb。
- 最佳活性温度为42°C。
- 温度高达50°C时仍有活性。
- 合成掺入修饰核苷酸，如Cy3-、Cy5-、罗丹明-、氨基-、荧光-标记的核苷酸。
- Applications
-
- 第一链cDNA合成，用于RT-PCR和实时RT-PCR。
- 合成cDNA，用于克隆和表达研究。
- 制备芯片研究用标记的cDNA探针。
- DNA标记(3)。
- 引物延伸法分析RNA (3)。
酶活性分析混合物：50 mM Tris-HCl (pH 8.3), 4 mM MgCl2 , 10 mM �TT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/ml [3 H]-dTTP, 0.4 mM �olyA・oligo(dT)12-18 。
50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM �DTA, 5 mM DTT, 0.1% (v/v) Triton X-100和50% (v/v)甘油。
- 抑制剂：金属螯合剂、无机磷酸盐、焦磷酸和多胺(2)。
- 失活：70°C加热10分钟。
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文献和实验相关专题 This method was submitted by Jim Hutchins from the University of Mississippi Medical Center. The following is a great RT-PCR protocol developed by Ignacio Rodriguez at the National Eye Institue, NIH. He gets
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center. The following is a great RT-PCR protocol developed by Ignacio Rodriguez at the National Eye Institue, NIH. He gets all the credit
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center. The following is a great RT- PCR protocol developed by Ignacio Rodriguez at the National Eye Institue, NIH. He gets all the credit
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