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- 规格:
50 tests
品牌介绍
全球的ELISPOT试剂盒生产商之一。公司创始人Schielen较早将PVDF膜板引入了ELISPOT,大大提高了ELISPOT的灵敏度。提供人、灵长类、大鼠、小鼠的T细胞、B细胞的ELISPOT kit,ELISA kit 及相关配套试剂。在ELISpot热卖产品炙热而璀璨的星河中,U-CyTech大鼠T细胞IL-4和IFN-γ完整试剂盒以独特而耀眼的光芒,成为了研究人员心中的皓月清凉。 U-CyTech是世界上最早将PVDF膜引进ELISpot的厂家,凭借20多年的经验和专业,U-CyTech团队研发了一系列包括细胞因子ELISA、B细胞和T细胞ELISpot检测、FluoroSpot试剂盒及其单克隆抗体等多种产品线,致力于帮助研究人员来了解最真实的免疫应答反应。 U-CyTech的ELISpot产品能灵敏地监测人类和动物细胞免疫反应,灵敏度可达百万分之一,是准确定量分析胞外细胞因子或分泌抗体的优质工具。 细胞免疫反应在新冠研究中尤为重要,而ELISpot可用于观察SARS-CoV-2 (COVID-19)对疫苗做出的真实免疫应答。例如,通过我们的T细胞 ELISpot产品Human IL-2和IFN-γ可以用于检测COVID-19疫苗接种前后的T细胞反应。
产品介绍
Human CD3/CD28 positive control in ELISPOT is useful as a polyclonal T-cell activator in the human ELISPOT assays. The vial contains two antibodies specifiec for human CD3 and CD28. A variety of different cytokine are elicited by this antibody combination including IFN-gamma, IL-2, IL-4, IL-5, IL-10 and IL-13. The recommended working concentration is 100x in culture medium containing > 10E4 human PBMC/ml. Upon ordering, you will receive one vial which is sufficient to analyze 50 ELISPOT samples.
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Intracellular Immunofluorescent Staining for Flow Cytometry
MQ1-17H12 IL-4 PBMC anti-CD3 (10µg/ml, immobilized) + anti-CD28 (2µg/ml, soluble) + IL-2 (10ng/ml) + IL-4 (20ng/ml) (2d); IL-2 (10ng/ml) + IL-4 (20ng/ml) (3d) 2d/3d PMA (5ng/ml) + Ionomycin
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal









