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Background
p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T-antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian,
bladder, colon, lung, and melanoma.
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文献和实验Literary References
Kula� I, Demir �, B�y�kasik Y, Kutluk T, �ner A. TP53 Staining in Tissue Samples of Chronic Lymphocytic Lymphoma Cases: An Immunohistochemical Survey of 51 Cases. Turkish journal of haematology: official journal of Turkish Society of Haematology. 2016 May 12.
Use of Inverse PCR to Clone cDNA Ends
Since the first report on complementary DNA (cDNA) cloning in 1972 (1 ), the technology has been developed into a powerful and universal tool in the isolation, characterization, and analysis of both eukaryotic and prokaryotic genes
Clone Genes From a Phage Library
, LB tet, LB Kan, LB amp; Top agar: NZY or TB plus 0.7 percent agar autoclave); Cells: XLBlue MR strain, which is tetracycline resistant (on an F prime element; 25 micrograms/ml tet), and the SolR strain, which is kanamycin resistant (use 25 micrograms
As more and more genes are cloned and sequenced, it is apparent that nearly all genes are related to other genes. Similar genes are grouped into families. Examples of gene families include the collagen, globin, and myosin gene families
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