p-Fect[Cationic Lipid]

Thermodynamics of Lipid Interactions with Cell-Penetrating Peptides

on particular CPP properties. Protocols include vesicle preparation, lipid quantification, and analysis of membrane leakage, lipid polymorphism (31 P NMR), and membrane binding (isothermal titration calorimetry). Using these protocols, a major difference

LIPID EXTRACTION

  PROCEDURE 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) 2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid

脂质 A lipid A

脂质 A lipid A 以葡萄糖胺、磷酸、脂肪酸为主要成分的糖脂质，是内毒素的活性中心部分。