拓扑异构酶Ⅱ(TOP2)检测试剂盒(酶联免疫吸附试验法)DN
A topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme- 询价
- Cloud-Clone
- 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
- SEA792Ra
- 美国
- 2025年11月05日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
南京赛泓瑞
- 检测范围：
0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Rattus norvegicus (Rat)
- 样本：
Tissue homogenates, cell lysates and other biological fluids
- 规格：
48T/96T
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文献和实验Topoisomerase I and II Activity Assays
DNA topoisomerases I and II (top1 and top2, respectively) are ubiquitous enzymes that play an essential role in transcription, replication, chromosome segregation, and DNA repair. The basic enzymatic reaction of topoisomerases
Assaying Topoisomerase II Checkpoints in Yeast
Topoisomerase II activity is crucial to maintain genome stability through the removal of catenanes in the DNA formed during DNA replication and scaffolding the mitotic chromosome. Perturbed Topo II activity causes defects in chromosome
(topoisomerase)是一类改变DNA拓扑性质的酶。在体外可催化DNA的各种拓扑异构化反应，而在生物体内它们可能参与了DNA的复制与转录。在DNA复制时，复制叉行进的前方DNA分子部分产生有正超螺旋，拓扑酶可松驰超螺旋，有利于复制叉的前进及DNA的合成。DNA复制完成后，拓扑酶又可将DNA分子引入超螺旋，使DNA缠绕、折叠，压缩以形成染色质。DNA拓扑异构酶有Ⅰ型和Ⅱ型，它们广泛存在于原核生物及真核生物中。表16－3 表16－3 大肠杆菌和真核生物中的拓扑异构酶�
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