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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
南京赛泓瑞
- 检测范围:
0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
- 检测方法:
ELISA
- 应用:
ELISA
- 适应物种:
Mus musculus (Mouse)
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 规格:
48T/96T
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文献和实验的PCR端粒酶活性检测试剂盒基于同样的的原理,利用银染技术检测端粒酶的活性。阳性结果在凝胶电泳上显示相隔6bp 的梯状条带,条带的多、寡、深或浅表示端粒酶活性的大小。TRAP-银染法保留了传统TRAP 法灵敏度高、特异性强等优点,避免了同位素的放射污染。同时,还增设了内标准物,提供阳性对照品,优化了引物设计,使PCR效果进一步提高,使之能够高灵敏的检测到细胞和组织提取物中端粒酶的活性。 TRAP-银染法端粒酶活性检测试剂实验方法 试剂盒组份 组分 50 次实验量
with a transmembrane anchor, but after translocation across the membrane of the endoplasmic reticulum, the ecto-domain of the protein is cleaved and covalently linked to a preformed GPI anchor by a specific transamidase enzyme. At least one human disease, paroxysmal
., Hessenberger, M., … & Brügger, B. (2021). Inositol triphosphate-triggered calcium release blocks lipid exchange at endoplasmic reticulum-Golgi contact sites. Nature Communications, 12(1), 2673. 2.Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy
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