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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
南京赛泓瑞
- 检测范围:
0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
- 检测方法:
ELISA
- 应用:
ELISA
- 适应物种:
Homo sapiens (Human)
- 样本:
Tissue homogenates and other biological fluids.
- 规格:
48T/96T
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文献和实验双特异抗体(Bispecific antibody)的简介和制作方法
receptors. It was observed recently that human antibodies of the IgG4 type can exchange their halves with each other, potentially creating antibodies with dual specificity [2]. However, the biological relevance of this observation remains obscure
液→检测。2、竞争法(检测抗体)抗原包被、封闭→加入一抗/检测样品→加入Eu3+标记的二抗→加入增强液→检测。三 、传统TRFIA 的改进1、酶放大的时间分辨荧光免疫分析法Papanastsiou-Diamandi等[5]报道这种分析技术,其原理与常规方法完全不同。本方法以碱性磷酸酶催化底物5- 氟水杨酸磷酸酯(FSA),FSA 与稀土离子Tb3+ 作用生成FSA-Tb3 + -EDTA 三元复合物。结合后的Tb3+在紫外光激发下可发出很强的荧光信号。不加增强液即可在时间分辨荧光仪上测量Tb
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