前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 产品概述

本48T/96T规格的前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1（STEAP2，货号 SEH122Hu）由Cloud-Clone提供，运用酶联免疫吸附法（ELISA）完成Tissue、homogenates,、cell、lysates、and、other、biological、fluids.样本的目标物定量，方法成熟、结果可重复。

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 检测原理

检测体系为酶联免疫吸附法（ELISA）——包被抗体捕获目标分子，酶标试剂产生可测信号，信号强度随浓度递增，配合标准曲线实现定量分析。

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 核心参数

STEAP2 靶点背景与检测意义

STEAP2（NCBI Gene ID 261729，官方基因名 STEAP2）。This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro.

本试剂盒以 STEAP2 为检测目标，适用于上述通路/功能相关的科研定量分析。

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 适用样本与处理

本试剂盒适用于 Tissue、homogenates,、cell、lysates、and、other、biological、fluids.。样本采集后请按下列要点处理：

• 血清：采血后室温凝固 30 min，离心取上清，避免溶血；
• 血浆：使用合适抗凝剂采集，及时离心分离；
• 细胞培养上清：离心去除细胞碎片后取上清；
• 组织匀浆：按质量体积比加入裂解/匀浆缓冲液，离心取上清。

样本如不能立即检测，请分装后低温保存，避免反复冻融。

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 操作流程

1. 试剂与样本平衡至室温，按随盒技术资料配制标准品、洗涤液及工作液；
2. 设置标准品孔、样本孔与空白孔，加入相应液体；
3. 孵育后洗板，加入酶标试剂再次孵育并洗涤；
4. 加入显色底物避光反应，加入终止液终止；
5. 于酶标仪读取吸光度（常规 450 nm），计算结果。

结果计算与标准曲线

以标准品浓度为横坐标、吸光度值为纵坐标绘制标准曲线（推荐四参数或对数拟合），将样本 OD 值代入求得浓度；超出曲线上限的样本请稀释后复测，结果乘以稀释倍数。

注意事项

• 本品仅供科研使用，不可用于临床诊断；
• 试剂请按 试剂盒 2-8℃ 避光保存，不得冷冻；复溶后的标准品请按要求分装并于 -20℃ 保存，避免反复冻融。；
• 不同批号组分请勿混用，洗板应充分以保证重复性；
• 显色时间需严格控制，终止后请及时读数。

质量控制与随附文件

产品出厂前经批间一致性、回收率与线性等质控项目检测，随盒提供质检报告（COA）与使用技术资料，检测范围与最低检出限以该批次 COA 标注为准。

前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1 常见问题

Q1：前列腺六跨膜表皮抗原2(STEAP2)检测试剂盒(酶联免疫吸附试验法)IPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1适合检测哪些样本？
A：适用于 Tissue、homogenates,、cell、lysates、and、other、biological、fluids.，其他样本类型建议先做预实验验证。

Q2：适用种属有哪些？
A：Homo sapiens (Human)，跨种属使用请先进行验证。

Q3：试剂盒如何保存、有效期多久？
A：试剂盒 2-8℃ 避光保存，不得冷冻；复溶后的标准品请按要求分装并于 -20℃ 保存，避免反复冻融。未开封 2-8℃ 条件下有效期 6 个月，具体以标签及质检报告（COA）为准。

参考文献（来源：PubMed）

[1] Zanvit P et al. Antitumor activity of AZD0754, a dnTGFβRII-armored, STEAP2-targeted CAR-T cell therapy, in prostate cancer. The Journal of clinical investigation. 2023. PMID:37966111

[2] Burnell SEA et al. STEAP2 Knockdown Reduces the Invasive Potential of Prostate Cancer Cells. Scientific reports. 2018. PMID:29674723

[3] Zhang D et al. STEAP2 promotes osteosarcoma progression by inducing epithelial-mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2. Cancer biology & therapy. 2022. PMID:36316642

靶点背景（来源：NCBI Gene）

STEAP2（NCBI Gene ID 261729，官方基因名 STEAP2，STEAP2 metalloreductase）。This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro.