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胰酶,Trypsin EDTA 1X

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  • ¥220
  • 康宁
  • 25-053-CI
  • 2025年07月16日
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      研卉生物

    • 英文名

      Trypsin EDTA 1X

    • 规格

      100 mL

    康宁cellgro胰酶,Trypsin EDTA 1X 25-053-CI

    Technical Advantage: Product manufactured under industry-leading standards for low endotoxin content and superior results.

    Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin. This product contains trypsin at a concentration of 0.25% and 2.21 mM EDTA.
    康宁cellgro培养基大全
    10-010-CVR MEM, 1X w/ Earle's salts & L-glut 6 x 500 mL
    10-013-CVR DMEM, 1X w/ 4.5 g/L glucose 6 x 500 mL
    10-014-CVR DMEM, 1X w/ 1 g/L glucose 6 x 500 mL
    10-016-CVR Iscove's DMEM, 1X w/ L-glut &HEPES 6 x 500 mL
    10-017-CVR DMEM, 1X w/ 4.5 g/L glucose 6 x 500 mL
    10-022-CVR Alpha MEM, 1X 6 x 500 mL
    10-040-CVR PMI 1640, 1X w/ L-glut 6 x 500 mL
    10-060-CVR Medium 199 (Mod), 1X 6 x 500 mL
    10-080-CVR Ham's F-12 Medium, 1X 6 x 500 mL
    10-092-CVR DMEM/ F-12 50/50 Mix, 1X w/ L-glut & HEPES 6 x 500 mL
    15-013-CVR Dulbecco's Modification of Eagle's Medium (DMEM)
    with 4.5 g/L glucose and sodium pyruvate without L-glutamine
    6 x 500 mL
    15-017-CVR Dulbecco's Modification of Eagle's Medium (DMEM)
    with 4.5 g/L glucose without L-glutamine and sodium pyruvate
    6 x 500 mL
    15-040-CVR RPMI1640M without L-glutamine 6 x 500 mL
    15-090-CVR Dulbecco's Modification of Eagle's Medium/Ham's F-12 50/50 Mix without L-glutamine 6 x 500 mL
    21-020-CVR HBSS, 1X, w/ Ca & Mg 6 x 500 mL
    21-021-CVR HBSS, 1X w/o Ca & Mg 6 x 500 mL
    21-022-CVR HBSS, 1X w/o Ca, Mg, & p.. 6 x 500 mL
    21-031-CVR DPBS, 1x w/o Ca & Mg 6 x 500 mL
    21-040-CVR PBS, 1X w/o Ca and Mg 6 x 500 mL
    25-000-CIR Sodium pyruvate 100 mM Soltn. 6 x 100 mL
    10-009-CV MEM,含非必需氨基酸 6 x 500 mL
    25-025-CIR MEM Non-essential Amino Acids 100X Soltn 6 x 100 mL
    10-041-CVR PMI 1640, 1X w/ L-glut & HEPES 6 x 500 mL
    15-041-CVR PMI1640, with 25mM HEPES, without L-glutamine 6 x 500 mL
    25-005-CI L-GLUTAMINE 6 x 100 mL
    10-025-CVR F-12K Nutrient Mixture 6 x 500 mL
    16-405-CVR DMEM/F12 w/o phenol red 6 x 500 mL
    10-045-CVR Leibovitz's L-15 (Mod) 6 x 500 mL
    13-200-CV Grace's Insect Basal Medium (Vaughn), 1X 6 x 500 mL
    25-015-CI glutagro Supplement 1 x 100 mL
    25-037-CIR 45% Glucose 1 x 100mL
    25-051-CI 0.05% Trypsin/0.53 mM EDTA 6 x 100 mL
    25-053-CI 0.25% Trypsin/2.21 mM EDTA 6 x 100 mL
    25-055-CM Sterile WFI-Quality Cell Culture Grade Water 6 x 1 L
    25-055-CV Sterile WFI-Quality Cell Culture Grade Water 6 x 500 mL
    25-056-CI Cellstripper细胞消化液 6 x 100 mL
    25-060-CI HEPES Buffer 1M Soltn 6 x 100 mL
    25-072-CI Lymphocyte Separation Medium 1 x 100 mL
    25-072-CV Lymphocyte Separation Medium 500 mL
    25-800-CR Insulin-Transferrin-Selenium 1 x 10 mL
    40-300-CVR tansfectagro™ reduced Seum Media 6 x 500 mL
    40-215-CVR hybrigro SF 6 x 500 mL
    40-550-CVR Hepatocyte maintenace medium 500 mL
    50-003-PBR DMEM w/ 4.5g/L glucose, L-glut & Na pyruvate 1 x 10 L
    40-410-KIT stemgro hMSC Medium KIT
    40-202-CM ADCF CHO-2 medium 1 x 1L
    50-003-PCR DMEM w/ 4.5g/L glucose, L-glut & Na pyruvate 1 x 50 L
    50-010-PBR Minimum Essential Medium (MEM), Powder, with Earle’s salts and L-glutamine, without sodium bicarbonate 1 x 10 L
    50-010-PCR Minimum Essential Medium (MEM), Powder, with Earle’s salts and L-glutamine, without sodium bicarbonate 1 x 50 L
    50-020-PBR RPMI 1640, 1X w/ L-glut & HEPES, with L-glutamine, without sodium bicarbonate 1 x 10 L
    50-020-PCR PMI 1640, with L-glutamine, without sodium bicarbonate 1 x 50 L
    50-045-PBR Leibovitz's L-15, 干粉 1 x 10 L
    61-030-RM L-GLUTAMINE, powder 1 x 100 g
    90-091-PBR DMEM /F-12 50/50 Mix w/o L-Glut, w p.. and phenol 1 x 10 L
    90-091-PCR DMEM /F-12 50/50 Mix w/o L-Glut, w p.. and phenol 1 x 50 L
    91-001-MB 25L COLLECTION Container 1 x 25L
    91-002-MX 10L COLLECTION Container 1 x 10L
    91-100-30 Collection Container 50L 1 x 50L
    91-100-35 Collection Container 100L 1 x 100L
    91-200-01 1L Collection Container, GI 1 x 1L
    91-200-02 2L Collection Container 1 x 2L
    91-200-05 5L Collection Container GI 1 x 5L
    91-200-10 10L Collection Container GI 1 x 10L
    91-200-20 20L Collection Container GI 1 x 20L
    91-200-36 10L Collection Container 1 x 10L
    91-200-39 20L Collection Container 1 x 20L
    91-200-41 Bag: 500mL Collection Container 500ml
    91-200-42 Bag: 1L Collection Container 1 x 1L
    91-200-43 Bag: 5L Collection Container 1 x 5L
    91-200-45 Bag: 10L Collection Contianer, 1 x 10L
    91-200-47 Bag: 20L Collection Container, 1 x 20L
    91-200-48 Bag: 50L Collection Container, 1 x 50L
    91-200-50 50L Gusseted Bag w/PE film 1 x 1 bag
    91-200-75 RESERVED: Bag: Hype, 5L Trypsin 1 x 5L
    91-200-76 RESERVED: Bag: Hype, 5L Quench 1 x 5L
    91-200-77 RESERVED: Bag: Hype, 20L Media 1 x 20L
    91-200-78 20L Rocker Bag 1 x 20L
    91-200-79 10L Rocker Bag 1 x 10L
    91-200-80 2L Rocker Cell Culture Bag 1 x 2L
    91-200-81 50L Rocker Cell Culture Bag 1 x 50L
    91-200-82 100L Collection Bag 1 x 100L
    91-200-83 200L Collection Bag 1 x 200L
    91-200-84 500 mL Expansion Bag 1 x 500 mL
    91-200-85 1L Expansion Bag 1 x 1L
    91-200-86 3L Expansion Bag 1 x 3L
    91-200-87 5L Expansion Bag 1 x 5L
    91-200-88 10 -20mL Cyo Bag 1 x 10-20 mL
    91-200-89 30-70 mL Cyo Bag 1 x 30-70 mL
    91-200-90 55-100 mL Cyo Bag 1 x 55-100 mL
    91-200-91 80-190 mL Cyo Bag 1 x 80-190 mL
    91-300-15 50L Gusseted Tank Liner 1 x 50L
    91-300-20 TANK LINER 130L 1 x 130L
    91-300-25 100L Gusseted Tank Liner 1 x 100L
    91-300-30 TANK Liner200L 1 x 200L
    91-300-35 200L Gusseted Tank Liner 1 x 200L
    91-300-60 560L Tank Liner 1 x 560L
    91-300-80 TANK LINER 1090L 1 x 1090L
    91-700-00 TUBE SET C-Flex 3'x1/4" ID 1 x 1
    91-700-02 TUBE SET C-Flex 3'x1/4" ID 1 x 1
    91-700-04 TUBE SET C-Flex 3'x1/4" ID 1 x 1
    91-700-06 TUBE SET C-Flex 3'x3/8" ID w/1 1 x 1
    91-700-08 TUBE SET C-Flex 3'x3/8" ID w/M 1 x 1
    91-700-12 FILLING BELL ASSEMBLY 1 x 1
    46-013-CM 1 L PBS (Phosphate-Buffered Saline), 10x, pH 7.4, Liquid, without calcium, magnesium 6 x 1 L
    10-070-CVR 500 mL Ham's F-10 Medium with L-glutamine 6 x 500 mL

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    图标文献和实验
    相关实验
    • 【求助】EDTA配方??

      不要太懒哦 请教各位高手,如果在细胞的消化传代过程中,我想以EDTA替代胰酶对细胞进行消化的话,EDTA的配方???浓度??多谢!! 佳杰宝贝 EDTA可以代替胰酶吗? 黑糊糊2000 EDTA 不能代替trypsin,EDTA是螯合剂结合钙离子的,钙离子可以抑制胰酶的作用,所以采用胰酶EDTA一起来作用,当然EDTA 因为对螯合细胞结构之间的钙离子的作用,所以对细胞有一定程度的离散作用

    • EDTA-胰蛋白酶的配制

      问:请问哪位仁兄能告知一下EDTA-胰蛋白酶的配制。急用!多谢!答:1、胰酶是0.25%,这是质量体积比,就是说0.25g胰酶溶于100ml PBS,注意,尽量不要用水来溶,因为要保持渗透压。2、EDTA的工作液溶度是0.02%-0.1%,根据细胞消化的难易程度自己调整。EDTA并不是必须的,容易消化的细胞可不加。EDTA对细胞贴壁性有影响,因此使用时要甚重。如果影响贴壁,但消化时必须要用,那么在用完全培养基终止消化后,可离心弃上清,再加完全培养基培养,可去除EDTA。如果对贴壁没

    • 细胞消化胰酶的配制

      适用于,无师兄、师姐、非细心、单独开展细胞培养的实验者 对一般细胞 0.25% 胰酶(胰蛋白酶,Trypsin) 配方:100ml PBS,0.25g胰酶 步骤:1先配100mlPBS(1000mlPBS:8.0gNaCl,3.4785gPO4HNa2・12H2O /2.9gPO4HNa2 ,0.2gKCl,0.2gPO4H2K溶于1000ml蒸馏水) 2 称胰酶0.25g 3 加入PBS中,低速搅拌 〈4h冰浴中,或者4℃过夜 低速搅拌0.5h冰浴中

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