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- 文献和实验
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- 库存:
充足
- 供应商:
Kapa biosystems
- 规格:
6 x 80 µl
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
Summary: Illumina's Solexa Sequencing Technology
fragments of the size range of 150-200bp are isolated via gel extraction and amplified using limited cycles of PCR.Complete detailed protocols for DNA and small RNA library preparation can be found in the documents provided in the attachments to this post
Multiplex Illumina Sequencing Using DNA Barcoding
cell. The quality and quantity of purified library DNA can be evaluated by qPCR and Bioanalyzer analyses, respectively. DNA libraries are then ready for mixing and Illumina sequencing. When mixing libraries with severe primer dimer contamination
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