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- Kapa biosystems
- 美国
- KK8420
- 2025年12月08日
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
Background: mRNAs （messenger RNAs） comprise only a small percentage of all RNA species in a eukaryotic cell, in Neurospora usually ~ 1-6 % （Lucas et al., 1977; Sturani et al., 1979）。 For some applications like preparation of a cDNA library, target
, amphibian, insect and plant origins. For many applications elution of the mRNA from the beads is not required as the beads do not interfere with downstream enzymatic reactions. The bead-bound oligo (dT)25 can also function as a primer for RT
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