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充足
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Kapa biosystems
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96 rxn
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
DNA Repair and Solexa Library Construction 39. Repair DNA ends to generate blunt-ended DNA using the DNA End-Repair Kit (Epicentre). Combine: 1-34 µL of DNA (0.3 µg) 5 µL of 10X End-Repair
Preparation of mRNA for Expression Monitoring
and enzymes are supplied with the SuperScript II cDNA kit. Kit enzymes that are limiting may be ordered separately from Life Technologies.NOTE: All temperature‐controlled reactions are performed in an appropriate thermal cycler
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Mammalian Genomic DNA Miniprep Kit (Catalog Number G1N10) or equivalent. We recommend preparing DNA from the parent cell line that does not contain Target ID Library to use as a negative control for comparison with DNA from target-selected cells in PCR
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