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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 供应商:
hyclone
- CAS号:
SV30068.01
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文献和实验200,000 dpm samples in 300 µl IMP buffer. 4) To preclear samples, add 50 µl of control (normal rabbit serum, mouse isotype control, etc.) to each tube and 25 µl of Protein G slurry (shake to suspend slurry before pipetting) and incubate for 1 h at 4 C
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
upon the formation of an insoluble antibody-antigen complex. Rather, antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody (Figure
The Detection of Neomycin Phosphotransferase Activity in Plant Extracts
from the bacterial transposon Tn5, the gene product (E.C.2.7.1.95) transfers the γ phosphate group of ATP to a specific hydroxyl group of aminoglycosidic antibiotics. In this way, it detoxifies neomycin, kanamycin, G418, as well as paromomycin. Plants expressing
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